Journal: Microorganisms

Article Title: Interferon-Induced Transmembrane Protein 3 (IFITM3) Restricts PRRSV Replication via Post-Entry Mechanisms

doi: 10.3390/microorganisms13081737

Figure Lengend Snippet: Colocalization of PRRSV with early endosome marker EEA1 and late endosome marker LAMP1 at 1, 3, and 6 h after virus infection. ( a ) Colocalization of PRRSV with EEA1 at different time points. ( b ) Mean percent colocalization was estimated by manual thresholding using Image J version 1.53. For each time point, mean percent colocalization was calculated by randomly selecting five cells for each microscopic field. A greater percentage of colocalization occurred with EEA1 at 3 hpi as compared to 1 or 6 hpi. ( c ) The colocalization of PRRSV with LAMP-1 at different time points. ( d ) Only limited colocalization between PRRSV and late endosome/lysosome marker LAMP1 was observed at 3 h after virus infection. The images were captured using a confocal microscope at 40× magnification.

Article Snippet: Then at three time points—1 h, 3 h, or 6 h after infection, the cells were fixed with 3.8% formaldehyde for 10 min at RT, then washed in PBS for 5 min and treated with 0.2% Triton X-100 for 10 min, after which cells were again washed once and incubated with 5% goat serum in PBS (blocking buffer) for 1 h. Then cells were incubated with SDOW17 (FITC conjugated anti-PRRSV N) monoclonal Ab at 1:80 dilution, together with either anti-EEA1 Ab (Santa Cruz Biotechnology, sc-365652, Dallas, TX, USA) or anti-Lamp-1 Ab (Santa Cruz Biotechnology, sc-19992) conjugated to Alexa Fluor 647.

Techniques: Marker, Virus, Infection, Microscopy

Journal: Immunity

Article Title: The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis

doi: 10.1016/j.immuni.2018.08.016

Figure Lengend Snippet: Key Resources Table

Article Snippet: Anti-EEA1 antibody , Cell Signaling Technologies , 3288S; RRID: AB_2096811.

Techniques: In Vivo, Enzyme-linked Immunospot, Recombinant, Transfection, In Situ, Enzyme-linked Immunosorbent Assay, Selection, Software